Determination of glutathione reductase and glutathione S-transferase activities in human erythrocytes and lymphocytes
Dirasat Journal Country: Jordan
P-ISSN: 1026-3764
MeSH Terms: Glutathione Reductase
Broad Subjects: Noncommunicable Diseases, Glutathione Transferases
Citation: Walid A. Al Turk, Sadeq Othman, Determination of glutathione reductase and glutathione S-transferase activities in human erythrocytes and lymphocytes. Dirasat. 1986; 13 (11): 113-125
The activities of glutathione S-transferase [GST] and glutathione reductase [GSR] in human erythrocytes and lymphocytes In relation to number of cells, protein concentration and as a function of time were determined. A three minute period was found to be suitable for incubation for the two enzymes in both erythrocytes and lymphocytes. A concentration of 0.56x 10[8] cells/ml was found to be optimal for assaying GST and GSR in erythrocytes and concentrations of 0.13 and 0.21×10[6] cells/ml lymphocytes for assaying GST and GSR. respectively. The activities of GST and GSR were linear up to 2.0 and 1.3mg protein/ml in erythrocytes and up to 0.90 and 0.09mg protein/ml in lymphocytes, respectively
