Total proteins of tears, different protein fractions utilizing cellulose acetate electrophoresis an immunoglobulin content of tears were studied in 40 subjects [20 males, 20 females] in a group of normal adults. Their serum samples were also collected for the estimation of total proteins and protein fractions. Total proteins of tears in both sexes were significantly lower than serum total proteins. Electrophoretic fractionation of tear proteins, revealed the existence of serum albumin, tear albumin, alpha-globulin, beta- globulin and lysozyme. Immunoglobulin study of tears confirmed the earlier studies that IgA was the predominant one, followed by IgG [present in low concentrations in the whole group] . No sex difference was reported. IgE could be detected in four females only. IgM and IgD were not detected in this study