To assess nurses’ attitude towards oral care for elderly patients and their knowledge about denture hygiene for elderly denture wearers and also to identify the relationship between nurses’ knowledge, attitude, and their socio-demographics. A cross sectional study was conducted over a period of one month, during August 2010. Registered nurses working at the Gulf Medical College Hospital and Research Center [GMCHRC] were invited to participate in this study. The response rate was 100%. An 11-item questionnaire was prepared by the investigators and validated by three experts, including two specialized dentists and one senior nurse. It was then pretested by piloting with 10 nurses, who were not included in the study. The data collected were summarized using descriptive analysis and the association between the reported knowledge and attitude and the nurses’ socio-demographics, education level, years of experience and previous education on oral health was explored. The findings indicated that overall, local nurses had adequate knowledge related denture hygiene. The large majority of the participants reported a positive attitude towards oral care for the elderly. The results also revealed that only 55% received lectures on oral care, half of which had received them during their undergraduate nursing education, which indicates the need for continuing education and training of nurses in this area to prepare them for the challenge of caring for the oral hygiene needs of elderly patients. The study provided useful insights on the nurses’ attitude towards oral care for elderly patients and their knowledge about denture hygiene for elderly denture wearers. The results highlighted the need for continuing education and training of nurses in this area