A novel high performance liquid chromatographic method for determination of nystatin in pharmaceutical formulations by box–behnken statistical experiment design 
Iran. J. Pharm. Res. 2015;
14
(Supp.):
43-49 Shokraneh Farnaz ; Asgharian, Ramin; Abdollahpour, Assem; Ramin, Mehdi; Montaseri, Ali; Mahboubi, Arash
Islamic Azad University [IAU] ; , Pharmaceutical Sciences Branch ; , Department of Pharmaceutics ;
IJPR-Iranian Journal of Pharmaceutical Research Journal Country: Islamic Republic of Iran
ISSN: 1735-0328 ISSN E: 1726-6890 Type of Publication: Journal Article Type of Research: Experimental Studies
Keywords: Health Systems,Essential Medicines, Chromatography, High Pressure Liquid ,Pharmaceutical Preparations
Subjects: Nystatin
In this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box-Behnken experimental design. The method
was performed in the isocratic mode on a RP-18 column [30 [degree] C] using a mobile phase consisting of ammonium acetate 0.05 M buffer/ Methanol mixture [30: 70] and a flow-rate of 1.0 mL/min. The specificity, linearity, precision, accuracy, LOD and LOQ of the method were validated. The method was linear over the range of 5-500 microg/mL with an acceptable correlation coefficient [r [2] = 0.9996] . The method's limit of detection [LOD] and quantification [LOQ] were 0.01 and 0.025 microg/mL respectively. The results indicate that this validated method can be used as an alternative method for assay of nystatin
Citation: Farnaz Shokraneh ,Ramin Asgharian ,Assem Abdollahpour ,Mehdi Ramin ,Ali Montaseri ,Arash Mahboubi , A novel high performance liquid chromatographic method for determination of nystatin in pharmaceutical formulations by box–behnken statistical experiment design, Iran. J. Pharm. Res. 2015; 14 (Supp.): 43-49
Views : 0
