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  4. A novel high performance liquid chromatographic method for determination of nystatin in pharmaceutical formulations by box–behnken statistical experiment design

A novel high performance liquid chromatographic method for determination of nystatin in pharmaceutical formulations by box–behnken statistical experiment design

Authors

Shokraneh Farnaz
Asgharian, Ramin
Abdollahpour, Assem
Ramin, Mehdi
Montaseri, Ali
Mahboubi, Arash
Islamic Azad University [IAU] ; , Pharmaceutical Sciences Branch ; , Department of Pharmaceutics ;

Iran. J. Pharm. Res. 2015; 14 (Supp.): 43-49
IJPR-Iranian Journal of Pharmaceutical Research
Journal Country: Islamic Republic of Iran
P-ISSN: 1735-0328
E-ISSN: 1726-6890
Type of Publication: Journal Article
Type of Research: Experimental Studies
Keywords: Nystatin
Board Subjects: Health Systems, Chromatography, High Pressure Liquid ,Pharmaceutical Preparations
Citation: Farnaz Shokraneh ,Ramin Asgharian ,Assem Abdollahpour ,Mehdi Ramin ,Ali Montaseri ,Arash Mahboubi , A novel high performance liquid chromatographic method for determination of nystatin in pharmaceutical formulations by box–behnken statistical experiment design, Iran. J. Pharm. Res. 2015; 14 (Supp.): 43-49

Abstract English

In this study a novel High Performance Liquid Chromatography for the assay of nystatin in oral and vaginal tablets were optimized and validated using Box-Behnken experimental design. The method was performed in the isocratic mode on a RP-18 column [30 [degree] C] using a mobile phase consisting of ammonium acetate 0.05 M buffer/ Methanol mixture [30: 70] and a flow-rate of 1.0 mL/min. The specificity, linearity, precision, accuracy, LOD and LOQ of the method were validated. The method was linear over the range of 5-500 microg/mL with an acceptable correlation coefficient [r [2] = 0.9996] . The method’s limit of detection [LOD] and quantification [LOQ] were 0.01 and 0.025 microg/mL respectively. The results indicate that this validated method can be used as an alternative method for assay of nystatin

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