COVID-19 | Discover the latest peer-reviewed research articles from the Eastern Mediterranean Region
COVID-19 | Discover the latest peer-reviewed research articles from the Eastern Mediterranean Region
COVID-19 | Discover the latest publications published by WHO Regional Office for the Eastern Mediterranean
COVID-19 | Discover the latest publications published by WHO Regional Office for the Eastern Mediterranean

Virtual Health Sciences Library

  • Home
  • Search
  • Knowledge Base
  • Get a journal indexed in the Index Medicus
  • MyVHSL
  1. Home
  2. IMEMR
  3. Search Result
  4. A preliminary study on endothelial nitric oxide synthase intron 4a/b polymorphism in ulcerative colitis-associated colorectal cancer

A preliminary study on endothelial nitric oxide synthase intron 4a/b polymorphism in ulcerative colitis-associated colorectal cancer

Authors

Gaballah Hanaa Hibishy
Ismail, Saber Abdelrahman
Tanta University ; , Faculty of Medicine ; , Medical Biochemistry Department ;

Egypt. J. Biochem. Mol. Biol. 2015; 33 (2-Jan): 16-33
EJB-The Egyptian Journal of Biochemistry and Molecular Biology
Journal Country: Egypt
P-ISSN: 1687-1502
Type of Publication: Journal Article
Category: Humans, Female, Male, Middle Aged,
Country of Research: Egypt WHO Eastern Mediterranean Region
Type of Research: Clinical
Keywords: Nitric Oxide Synthase Type III
Broad Subjects: Health Systems, Polymorphism, Genetic ,Introns ,Colitis, Ulcerative ,Colorectal Neoplasms
Citation: Hanaa Hibishy Gaballah ,Saber Abdelrahman Ismail , A preliminary study on endothelial nitric oxide synthase intron 4a/b polymorphism in ulcerative colitis-associated colorectal cancer, Egypt. J. Biochem. Mol. Biol. 2015; 33 (2-Jan): 16-33

Abstract English

Cancer is one the most concerning complication of longstanding ulcerative colitis [UC] . This study was undertaken to investigate the possible association between eNOS gene intron 4 polymorphism and the development of UC-associated colorectal cancer. It was carried out on 120 subjects; distributed as follows: 40 patients with UC associated colorectal cancer, 40 patients with UC who did not develop colorectal cancer and 40 control subjects. Genotypes [aa, bb, ab] for eNOS gene intron 4 polymorphism were identified using amplified fragment length polymorphism PCR. Plasma nitrate and nitrite levels were used to estimate the amounts of endogenous nitric oxide formation using nitric oxide colorimetric assay kit. Our preliminary data revealed that, compared to the bb genotype, the aa and ab eNOS genotypes were significantly associated with increased risk of developing UC associated colorectal cancer. Meanwhile, in UC associated – colorectal cancer and UC patients groups, plasma NO levels were higher in patients with 4a4a/4a4b genotypes compared to those with bb genotype. It can be concluded from our preliminary study that a allele variant of eNOS intron 4 polymorphism may be associated with increased risk of development of UC associated colorectal cancer, however, large scaled studies are needed to verify these preliminary results

Full Text English

Subscribe to VHSL Newsletters

© 2020 WHO – EMRO