Background: A biofilm is a complex aggregation of microorganisms in which cells adhere to a surface and form colonies. Biofilms have been shown to develop on medical device surfaces like IUD.
A biofilm has greater resistance to environmental stresses, including antibiotics Objective: The aim of our study is isolation of microorganisms formed on the IUDs removed from complaining females attending the Gynecological Clinic of Zagazig University Hospitals, assessment of the ability of the identified bacterial isolates to form biofilm and finally testing the antibiotic susceptibility of the biofilm forming bacteria to different antibiotics in both the biofilm and planktonic forms
Methodology: This study includes 72 females visiting gynecological outpatient clinic in Zagazig University Hospitals for removing their intrauterine devices [IUDs] . The intrauterine devices were removed with aseptic techniques. All samples were subjected to the following, microscopic examination of direct smears stained with Gram stain, cultivation on the suitable culture media, identification of isolates by API. Assessment of biofilm formation among the bacterial isolates were done by tissue culture plate method. Antibiotic susceptibility testing for planktonic cells and for biofilm forming bacteria were done by broth microdilution method Results: This study showed that IUD cultures were positive in 70 [97.2%] women and negative in 2 [2.8%] women. Our study showed that the most common bacterial species isolated from 56 bacterial and mixed IUDs culture results were Coagulase negative staphylococci [32.1%], Escherichia coli [25.0%], Pseudomonas aeruginosa [21.4%], Klebsiella spp. [12.5%] and Staphylococcus aureus [9%] . In our study, we found that among 56 bacterial isolates, 10 [17.8%] were moderate biofilm forming bacteria, 37 [66.1%] were weak biofilm forming bacteria and 9 [16.1%] were non biofilm forming bacteria Results of testing antimicrobial susceptibility of biofilm forming bacteria to different antibiotics both in the planktonic and biofilm forms showed that biofilm cells had higher resistance than planktonic cells to these antibiotics
Conclusion: Infection of IUD by biofilm forming bacteria is relatively high. All the biofilm forming bacterial isolates in the biofilm form show higher resistance to all the antibiotics than their planktonic counterparts
Azza A. ElSharkawy ,Azza A. ElSharkawy ,Randa S. Abdel Latif ,
Biofilm forming bacteria isolated from intrauterine devices and their susceptibility to antibiotics,
Egypt. J. Med. Microbiol. 2017;
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