Prince of Songkla University ; , Faculty of Pharmaceutical Sciences ; , Department of Pharmacognosy and Pharmaceutical Botany ;
Pakistan Journal of Pharmaceutical SciencesJournal Country: Pakistan ISSN: 1011-601X Type of Publication: Journal Article Type of Research: Non Clinical Keywords: Health Systems,Essential Medicines, Glucosidases ,Diabetes Mellitus ,Medicine, East Asian Traditional ,Phytotherapy
Nineteen plants from Fabaceae family, which were used in Thai traditional medicine for treatment of diabetes, were determined of alpha-glucosidase inhibitory activity via enzymatic reaction.
this reaction, alpha-glucosidase was used as enzyme, which, reacted with the substrate, Rho-nitrophenol-D-glucopyranoside [pNPG] . After that the product, p-nitro phenol [pNP] will be occurred and observed the yellow colour at 405 nm. In this study, acarbose was used as positive standard which, inhibited this enzyme with IC  as 331 +/- 4.73 microg/ml. Caesalpiniapulcherrima leaves showed the highest activity with IC  as 436.97 +/- 9.44 microg/ml. Furthermore, Bauhinia malabarica leaves presented moderately activity with IC  as 745.08 +/- 11.15 microg/ml. However, the other plants showed mild to none activity of alpha-glucosidase inhibition. Accordingly, this study can support anti-diabetes of these plants in traditional medicine and it will be the database of the biological activity of Fabaceae plant
Sukanya Dej adisai ,Thanet Pitakbut ,
Determination of alpha-glucosidase inhibitory activity from selected Fabaceae plants,
Pak. J. Pharm. Sci. 2015;
1679-1683 Views : 0