A Simple, sensitive and accurate high-performance liquid chromatographic [HPLC] method for effective and specific analysis of Loxoprofen [LXP] in the mobile phase and human plasma was developed.
Effective chromatographic separation was attained on a Mediterranea Sea C18 column [250×4.6mm, 5um] with mobile phase containing acetonitrile and 0.01 M NaH2PO4 buffer [55: 45] by adjusting pH 6.5 with sodium dihydrogen phosphate buffer at a flow rate of 1ml/ min. Calibration ranges from 0.1ppm to 10 ppm with a coefficient of relation value [R2=0.999] by using a linear regression method and lower limit of quantification was 0.1ppm. The current method showed inter-day and intra-day accuracy and precision within the range of +/- 10%. % RSD was found to be less than 5 %. Analytical recovery was more than 90% which confirmed the reliability of current method. The proposed method was found appropriate for assessment of LXP in pharmacokinetic and bioequivalence study
Muhammad Farooq ,Muhammad Farooq ,Muhammad Harris Shoaib ,Rabia Ismail Yousuf ,Fahad Siddiqui ,Muhammad Hanif ,Shazia Naz ,Lubna Bashir ,
Development and validation of liquid chromatographic method for quantitative determination of Loxoprofen in mobile phase and in human plasma,
Pak. J. Pharm. Sci. 2018;
2629-2633 Views : 0