Human peripheral blood monocytes [HPBM] express HLA-DR antigen which is involved in antigen presentation processes. HLA-DR expression on HPBM is upregulated by interferon-gamma [IFN-gamma] .
looked at the effect of the glucocorticoid dexamethasone [DEX] on HLA-DR expression by HPBM cultured in serum free media using anti-human monoclonal antibodies conjugated with phycoerythin at 48 hours and under different exposure conditions. Results were assessed by immunofluorescence microscopy and flow activated cell sorter [FACS] analysis. DEX in a concentration of 1x 10[-6] M did not significantly alter HLA-DR expression on HPBM, however in the same concentration it significantly enhanced INF-gamma induced HLA-DR upregulation. Furthermore we looked at the effect of DEX on RSV infected monocytes detected by anti-RSV monoclonal antibodies conjugated with fluorescine isothiocyanate [FITC] and analysed by two colour FACS analysis. DEX at 1x10[-6] M significantly enhanced HLA-DR expression on virus infected monocytes but not non-infected cells. These data suggest that the interactions between glucocorticoids and monocyte immunoregulatory functions are more complex than previously thought and may not always be of immunosuppressive nature
Hisham Tarraf ,Mona El Falaki ,Samia Rizt ,
Effect of dexamethasone on HLA-DR expression on human peripheral blood monocytes,
J. Egypt. Med. Assoc. 1994;
195-207 Views : 0