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  4. Effect of human ovarian tissue vitrification/warming on the expression of genes related to folliculogenesis

Effect of human ovarian tissue vitrification/warming on the expression of genes related to folliculogenesis

Authors

Mofarahe Zahra Shams
Shahid Beheshti University of Medical Sciences ; , School of Medicine ; , Department of Biology and Anatomical Sciences ;

Iran. Biomed. J. 2015; 19 (4): 220-225
IBJ-Iranian Biomedical Journal
Journal Country: Islamic Republic of Iran
P-ISSN: 1028-852X
E-ISSN: 2008-823X
Type of Publication: Journal Article
Category: Adult, Female, Humans,
Type of Research: Clinical
Keywords: Vitrification
Board Subjects: Maternal, Reproductive and Child Health, Ovary ,Gene Expression ,Ovarian Follicle
Citation: Zahra Shams Mofarahe , Effect of human ovarian tissue vitrification/warming on the expression of genes related to folliculogenesis, Iran. Biomed. J. 2015; 19 (4): 220-225

Abstract English

Ovarian tissue cryopreservation is an alternative strategy to preserve the fertility of women predicted to undergo premature ovarian failure. This study was designed to evaluate the expression of folliculogenesis-related genes, including factor in the germline alpha [FIGLA] , growth differentiation factor-9 [GDF-9] , follicle-stimulating hormone receptor [FSHR] , and KIT LIGAND after vitrification/warming of human ovarian tissue. Human ovarian tissue samples were collected from five transsexual women. In the laboratory, the ovarian medullary part was removed by a surgical blade, and the cortical tissue was cut into small pieces. Some pieces were vitrified and warmed and the others were considered as non-vitrified group [control] . Follicular normality was assessed with morphological observation by a light microscope, and the expression of FIGLA, KIT LIGAND, GDF- 9, and FSHR genes was examined using real-time RT-PCR in both the vitrified and non-vitrified groups. Overall, 85% of the follicles preserved their normal morphologic feature after warming. The percentage of normal follicles and the expression of FIGLA, KIT LIGAND, GDF-9, and FSHR genes were similar in both vitrified and non-vitrified groups [P > 0.05] . Vitrification/warming of human ovarian tissue had no remarkable effect on the expression of folliculogenesis-related genes

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