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  4. Effects and mechanism of baicalin on apoptosis of cervical cancer HeLa cells in-vitro

Effects and mechanism of baicalin on apoptosis of cervical cancer HeLa cells in-vitro

Authors

Peng Yong
Fu, Zhan Zhao
Guo, Cong Shan
Zhang, Yan Xia
Di, Ya
Jiang, Bin
Li, Qing Wang
Yanshan University ; , College of Electrical Engineering ; College of Environment, Chemical Engineering, Department of Biomedical Engineering ; Department of Biological Engineering

Iran. J. Pharm. Res. 2015; 14 (1): 251-261
IJPR-Iranian Journal of Pharmaceutical Research
Journal Country: Islamic Republic of Iran
P-ISSN: 1735-0328
E-ISSN: 1726-6890
Type of Publication: Journal Article
Type of Research: Experimental Studies
Keywords: Flavonoids
Board Subjects: Health Systems, Apoptosis ,Hela Cells ,Uterine Cervical Neoplasms ,In Vitro Techniques ,Caspase 3
Citation: Yong Peng ,Zhan Zhao Fu ,Cong Shan Guo ,Yan Xia Zhang ,Ya Di ,Bin Jiang ,Qing Wang Li , Effects and mechanism of baicalin on apoptosis of cervical cancer HeLa cells in-vitro, Iran. J. Pharm. Res. 2015; 14 (1): 251-261

Abstract English

The objective of this study was to observe the apoptosis-inducing effect and mechanism of baicalin on human cervical cancer HeLa cells. The inhibitory effect of baicalin on the growth of HeLa cells was measured by MTT assay, and cell proliferation and migration was analyzed by cell scratch assay. Morphological changes of apoptotic cells were viewed by the light microscope and electron microscope, and cell growth arrest was confirmed by flow cytometry. Moreover, Western blot was used for investigating the expression of apoptosis related proteins; spectrophotometry was used to examine Caspase-3 activation. Our results showed that baicalin could inhibit the proliferation of HeLa Cells via induction of apoptosis in a time and dose-dependent manner [P<0.01] . Apoptotic signaling induced by baicalin was characterized by up-regulating Bax, Fas, FasL and Caspase-8 protein expression, and down-regulating of Bcl-2 protein expression. These results indicated that baicalin-induced apoptosis involved activation Caspase-3 in HeLa cells through the intracellular mitochondrial pathway and the surface death receptor pathway

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