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  4. Effects of peptone supplementation in different culture media on growth, metabolic pathway and productivity of CHO DG44 cells; a new insight into amino acid profiles

Effects of peptone supplementation in different culture media on growth, metabolic pathway and productivity of CHO DG44 cells; a new insight into amino acid profiles

Authors

Davami Fatemeh
Eghbalpour, Farnaz
Nematollahi, Leila
Barkhordari, Farzaneh
Mahboudi, Fereidoun
Pasteur Institute of Iran ; Biotechnology Research Center,

Iran. Biomed. J. 2015; 19 (4): 194-205
IBJ-Iranian Biomedical Journal
Journal Country: Islamic Republic of Iran
P-ISSN: 1028-852X
E-ISSN: 2008-823X
Type of Publication: Journal Article
Type of Research: Non Clinical
Keywords: Peptones
Broad Subjects: Health Systems, Culture Media ,CHO Cells ,Recombinant Proteins ,Growth ,Metabolic Networks and Pathways ,Amino Acids
Citation: Fatemeh Davami ,Farnaz Eghbalpour ,Leila Nematollahi ,Farzaneh Barkhordari ,Fereidoun Mahboudi , Effects of peptone supplementation in different culture media on growth, metabolic pathway and productivity of CHO DG44 cells; a new insight into amino acid profiles, Iran. Biomed. J. 2015; 19 (4): 194-205

Abstract English

The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development; however, their role on metabolic pathway is not well understood. In the present study, the effect of different concentration of peptones on a recombinant Chinese hamster ovary [CHO] cell line grown in three serum-free suspension cultures was determined. Six peptones of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes. In optimized feeding strategies, increases of 136% and 159% in volumetric productivity [for a low-nutrient culture media] and 55% [for a high-nutrient culture media] were achieved. Furthermore, particular sources of peptones with specific amino acid profile developed preferential results for each different culture medium. Two peptones, SoyA2SC and SoyE-110, were the only hydrolysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium. The amino acid profile of peptones may provide clues to identify the most effective feeding strategies for recombinant CHO cells

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