Three commercially available ELISA kits and two confirmatory tests were evaluated for human immunodeficiency virus [HIV] [screening and confirmation] on sera from three groups of Saudi. Egyptian.
and other nationalities:  2580 new recruits and contractors;  114 carriers of hepatitis B surface antigen; and  82 patients attending the venereal disease [VD] clinic. In all the three groups screened, anti-HIV reactivity was reported by Abbott enzyme linked immunosorbent assay [ELISA] screening kit in 24 samples [0.86%] , Boehringer Mannheim Enzymun- test anti-HIV 1+2 kit in 23 samples, [0.83%] and Organon tecknika, Vironostika anti-HTLV-III kit in 21 samples [0.076%] and the difference between the three ELISA screening assays was slight, however, only two samples among group  were definitely positive [0.07%] as judged by a confirmatory Immunofluorescent test and the Western blot test. The two confirmed cases were non-arabs and. left the country immediately. The borderline cases and false-positive cases were subjected to follow-up examination extended for 6 monthes without confirmation and considered negative. The high rate of reactivity and slight variability of the results obtained by different commercial ELISA assays strongly indicates that repeat testing by ELISA assay does not replace the essential confirmatory step
M. E. Berry ,M. S. Wasfi ,
Evaluation of enzyme-linked immunosorbent assay ELISA in the diagnosis of human immunodeficiency virus,
Egypt. J. Med. Microbiol. 1994;
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