Thistudy represents the first report on the chemical composition and biological activity of Trifolium angustifolium var. angustifolium. The major components of the essential oil were identified as hexatriacontene [23.0%] , arachidic acid [15.5%] and alpha -selinene [10.0%] . The main constituents of the fatty acid obtained from the petroleum ether extract were identified as palmitic acid [29.8%] , linoleic acid [18.6%] and oleic acid [10.5%] . In particular, the water extract exhibited higher activity than alpha -tocopherol and BHT, which were used as standards in the ABTS cation radical scavenging assay and indicated higher inhibitory effect against acetylcholinesterase enzyme than the reference compound, galanthamine but exhibited weak activity in beta -carotene bleaching, DPPH-free radical scavenging, and cupric-reducing antioxidant capacity assays. The petroleum ether extract exhibited higher activity than alpha -tocopherol which was used as standard in the beta -carotene bleaching method at concentration 100 micro g/mL. The acetone extract exhibited higher activity than alpha -tocopherol which was used as standard cupric reducing antioxidant capacity [CUPRAC] method at 100 micro g/mL concentration The acetone and methanol extracts were active on all microorganisms tested with a small zone diameter indicating weak activity