To determine the frequency of Hyperprolactinemia in subfertile women presenting to Nishtar Hospital Multan. Case series study. This study was carried out in Gynae and Obst. Outpatient Department and Gynae and Obst. Units of Nishtar Hospital, Multan from Oct.2009 to March 2010. A total of 111 Patients with subfertility were selected which were fulfilling inclusion criteria. The prevalence of HPR in subfertile women at Nishtar Hospital Multan is 31.53%, and it is more common in women with primary subfertility i. e.23.42% than in secondary subfertility where it is 8.10%. It is concluded that hyperprolactinemia [HPR] leads to anovulation which is a main cause of subfertility, more common among women with primary subfertility than secondary subfertility