A method for detecting large numbers of isolates of enterotoxigenic bacteria is described in which the genes encoding the heat-labile [LT] enterotoxin is detected rather than the toxins themselves.
Biotin labeled fragments of DNA encoding the [LT] toxin was used as a hybridization probe derived from Escherichia coli. This probe hybridize with homologous DNA sequences in enteric bacterial colonies isolated from infants with diarrhea grown and Lysed on nylon filters. The [LT] probe detected  E. coli isolates,  Salmonella typhimurium,  Campylobacter jejuni,  Aeromonas spp. and  Shigella spp. None of the enteroaggregative E. coli [EAEC] and enterohemomhagic E. coli [EHEC] isolates hybridized with the [LT] probe. The DNA hybridization assay is useful and sensitive for identifying a large number of enteric bacteria isolated from clinical specimens
Layla K. Mehdi ,Layla K. Mehdi ,Zaki Abdul Ghani ,
Identification of enterotoxigenic bacteria by dna colony hybridization using heat labile enterotoxin gene probe,
Iraqi J. Med. Sci. 2003;
345-347 Views : 0