Seven commonly used broad spectrum antibiotics were screened for their immunomodulatory role through a simple immunoassay. Dimethylsulfoxide [DMSO] and colchicines were used as positive controls.
The probes in varying concentrations were incubated with standardized suspension of human peripheral blood lymphocytes which then were tested for their residual sheep erythrocyte rosette forming ability. Untreated preparations of lymphocytes were used as checks. Extent of inhibition achieved per unit increase in the concentration was tested in separate experiments for each probe utilizing polynominal regression approach of analysis of variance in Randomized Complete Block Design. All antibiotics tested were found to be immunomodulatory. Exposure to six of them resulted in decrease in E - rosette forming ability of the lymphocytes. Antibiotics in the descending inhibitory order were tetracycline, amphoterin B, neomycin, cephradine, amoxicillin and azteranam. Increase in concentration of nystatin demonstrated a favorable effect upon rosette formation
R. Vahidy ,F. Haider ,
Immunomodulatory effect of antibiotics evaluated through E-rosette assay,
Pak. J. Pharmacol. 1993;
41-51 Views : 0