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  4. Immunomodulatory effects of bee venom in human synovial fibroblast cell line

Immunomodulatory effects of bee venom in human synovial fibroblast cell line

Authors

Mohammadi Ebrahim
Shahid Beheshti University of Medical Sciences ; Kurdistan University of Medical Sciences, Kurdistan Environmental Health Research Center ; , Department of Toxicology and Pharmacology ;

Urol. Ann. 2015; 14 (1): 313-320
IJPR-Iranian Journal of Pharmaceutical Research
Journal Country: Islamic Republic of Iran
P-ISSN: 1735-0328
E-ISSN: 1726-6890
Type of Publication: Journal Article
Category: Humans,
Type of Research: Experimental Studies
Keywords: Bee Venoms
Board Subjects: Health Systems, Immunologic Factors ,Immunomodulation ,Fibroblasts ,Cell Line ,Smoking ,Arthritis, Rheumatoid ,Synovial Fluid
Citation: Ebrahim Mohammadi , Immunomodulatory effects of bee venom in human synovial fibroblast cell line, Urol. Ann. 2015; 14 (1): 313-320

Abstract English

As in Iranian traditional medicine, bee venom [BV] is a promising treatment for the rheumatoid arthritis [RA] which is considered as a problematic human chronic inflammatory disease in the present time. Smoking is considered to be a major risk factor in RA onset and severity. The main aim of this study is to investigate the effects of BV on cigarette smoke-induced inflammatory response in fibroblast-like synoviocytes [FLS] . Cytotoxicity of cigarette smoke condensate [CSC] and bee venom were determined by the tetrazolium [MTT] method in cultured synovial fibroblastes. The expression of interleukin-1 beta and sirtuin1 mRNA were analyzed by SYBR green real-time quantitative PCR. Differences between the mean values of treated and untreated groups were assessed by student t-test. Based on MTT assay, CSC and BV did not exert any significant cytotoxic effects up to 40 micro g/mL and 10 micro g/mL, respectively. Our results showed that interleukin-1 beta mRNA level was significantly up-regulated by CSC treatments in LPS-stimulated synoviocytes in a dose-dependent manner. Conversely, the expressions of IL-1 beta and Sirt1 were up-regulated even in lower concentrations of BV and attenuated at higher concentrations. Also, BV attenuated the CSC-induced and LPS-induced inflammatory responses in synovial fibroblasts. Our results support the epidemiological studies indicating pro-inflammatory effects of CSC and anti-inflammatory effects of BV on FLS cell line

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