This study was designed to evaluate the role of nerve growth factor [NGF] and transforming growth factor-beta2 [TGF-beta2] in the pathogenesis of insulin dependant diabetes mellitus [IDDM] .
study was done on diabetic pediatric patients regularly visiting the pediatric out-patient clinic of diabetes at El Minia university hospital. Nerve growth factor and transforming growth factor-Beta2 levels were measured in the serum of the patients with insulin dependant diabetes mellitus [n = 20] and the resulted values were compared to those of age-matched normal subjects [n = 13] . All IDDM patients were on multiple daily insulin injections and on no other medications. Both nerve growth factor and transforming growth factor-Beta2 were measured by a solid-phase sandwich, two-site, enzyme-Iinked immunoassay [ELISA] . The results proved that serum nerve growth factor levels were significantly elevated in insulin dependant diabetes mellitus patients [1798 +/- 296 pg/ml] compared to those of age-matched control [687 +/- 145pg/ml] ; [p < 0.001] . Serum transforming growth factor-Beta2 levels were significantly decreased in IDDM patients [294 +/- 48pg/ml] when compared with the healthy control [577 +/- 74pg/ml] ; [p <.001] . There was no correlation between the levels of nerve growth factor and transforming growth factor-Beta2 Conclusion: We concluded that an increase in nerve growth factor and decrease in transforming growth factor-beta2 levels were present in patients with insulin dependant diabetes mellitus and that both cytokines may play independent roles in the pathogenesis of this disease
Samir Tamer Abdullah ,Samir Tamer Abdullah ,Essmat Abdelaziz Elsharkawi ,
Plasma levels of nerve growth factor and transforming growth factor-beta2 in insulin dependant diabetes mellitus in children,
Alex. J. Pediatr. 2003;
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