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  4. Quantification of phenolic compounds, evaluation of physicochemical properties and antioxidant activity of four date [Phoenix dactylifera L. ] varieties of Oman

Quantification of phenolic compounds, evaluation of physicochemical properties and antioxidant activity of four date [Phoenix dactylifera L. ] varieties of Oman

Authors

Al Harthi S. S.
Mavazhe, A.
Al Mahroqi, H.
Khan, S. A.
Oman Medical College ; , Department of Pharmacy ;

J. Taibah Univ. Med. Sci. 2015; 10 (3): 346-352
Journal of Taibah University Medical Sciences
Journal Country: Saudi Arabia
P-ISSN: 1658-3612
Type of Publication: Journal Article
Country of Research: Oman WHO Eastern Mediterranean Region
Type of Research: Non Clinical
Keywords: Phoeniceae / Chemistry
Broad Subjects: Health Systems, Phenols ,Antioxidants ,Physicochemical Phenomena ,Chromatography, High Pressure Liquid
Citation: S. S. Al Harthi ,A. Mavazhe ,H. Al Mahroqi ,S. A. Khan , Quantification of phenolic compounds, evaluation of physicochemical properties and antioxidant activity of four date [Phoenix dactylifera L. ] varieties of Oman, J. Taibah Univ. Med. Sci. 2015; 10 (3): 346-352

Abstract English

This study aimed to evaluate the antioxidant activity and to quantify the phenolic compounds by colorimetric and High Performance Liquid Chromatography [HPLC] analytical methods of four dates palm varieties cultivated in Oman. The sun-dried mature fruits [Tamer stage] of Bunarinja, Khasab, Fard and Khalas date cultivars were collected from Al-Dakhiliya and Al-Sharqiya regions, Oman at the beginning of the 2013 harvest season. The antioxidant activities of date fruits were investigated by 1, 1 diphenyl-2-picrylhydrazyl [DPPH] free radical scavenging method. Folin-Ciocalteau colorimetric and HPLC methods were used to determine the total phenolic content [TPC] and to quantify the phenolic acids, respectively in the hydroalcoholic date fruit extracts. A preliminary HPLC analysis of the date fruits showed the presence of phenolic acids like Gallic acid, Vanillic acid, Caffeic acid, p-Coumaric and Syringic acid in different proportions. The TPC of all four varieties ranged from 32.24 mg to 35.84 mg Caffeic acid equivalents/100 g of fresh weight. All varieties exhibited significant free radical scavenging activity [28.78-70.62%] in a concentration dependent manner. The present study confirms that Omani dates are a rich source of phenolic compounds and posses good antioxidant properties. HPLC also revealed the Gallic acid as the predominant phenolic acid in all date cultivars

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