Pooled buffalo semen of motility more than 60% was used for the freezing process. Selected antibiotics were added in combination to the non-glycerolated part of buffalo milk and tris-yolk diluent.
Cephapirin with Amikacin or Gentamycin were used in different concentrations. Penicillin G sodium [1000 ug/ml] and Streptomycin [1000 ug/ml] were used as control. The data demonstrated that the combined addition of antibiotics did not affect the sperm viability. The viability index [VI] was maximum with Cephapirin [1000 ug/ml] + Gentamycin [750 ug/ml] in case of buffalo milk and tris-yolk diluents. The VI in buffalo milk diluent was higher than in tris diluent. The Fructolytic index [FI] was higher in samples treated with combined antibiotics than in control samples or in samples with Penicillin plus Streptomycin
M. A. Aziz ,
Semen picture studies on the use of combined selected antibiotics in different extenders of buffalo frozen semen,
Alex. J. Vet. Sci. 1994;
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