COVID-19 | Discover the latest peer-reviewed research articles from the Eastern Mediterranean Region
COVID-19 | Discover the latest peer-reviewed research articles from the Eastern Mediterranean Region
COVID-19 | Discover the latest publications published by WHO Regional Office for the Eastern Mediterranean
COVID-19 | Discover the latest publications published by WHO Regional Office for the Eastern Mediterranean

Virtual Health Sciences Library

  • Home
  • Search
  • Knowledge Base
  • Get a journal indexed in the Index Medicus
  • MyVHSL
  1. Home
  2. IMEMR
  3. Search Result
  4. Study of the antioxidant effects of Eremostachys laciniata rhizome extracts in isolated rat hepatocytes

Study of the antioxidant effects of Eremostachys laciniata rhizome extracts in isolated rat hepatocytes

Authors

Vaez Haleh
Arab, Mojgan
Delazar, Abbas
Eghbal, Mohammad Ali
Tabriz University of Medical Sciences ; , Faculty of Pharmacy ; Drug Applied Research Centre, Department of Pharmacology and Toxicology ;

Trends Pharm. Sci. 2015; 1 (3): 139-148
TIPS-Trends in Pharmaceutical Sciences
Journal Country: Islamic Republic of Iran
P-ISSN: 2423-3722
E-ISSN: 2423-5652
Indexing Status : In Process
Citation: Haleh Vaez ,Mojgan Arab ,Abbas Delazar ,Mohammad Ali Eghbal , Study of the antioxidant effects of Eremostachys laciniata rhizome extracts in isolated rat hepatocytes, Trends Pharm. Sci. 2015; 1 (3): 139-148

Abstract English

Eremostachys laciniata, having rich flavonoids content, is expected to have a considerable antioxidant effects. We used ACMS [Accelerated cytotoxic or protective mechanism screening technique] to evaluate possible antioxidant effect of E. laciniata rhizome against oxidative cell damages induced by different types of oxidative stress such as iron-8-hydroxyquinolin [IQ] complex and copper in freshly isolated liver cells. The extracts were prepared with n-hexane, dichloromethane and methanol. Hepatocytes were isolated from male Sprague-Dawley rats by a two-step collagenase perfusion. Cell viability was measured by trypan blue exclusion method. DPPH [2, 2-diphenyl-1-picrylhydrazyl] assay was used to evaluate the antioxidant activity. ROS formation was measured by using DCFDA [2, 7-dichlorofluorescin diacetate] probe, mitochondrial membrane potential [MMP] was assessed by rhodamine 123 fluorescence and lipid peroxidation was determined by thiobarbituric acid reactive substances [TBARS] assay. The MET extract was demonstrated to possess a significant radical scavenging activity [RC50%=0.212] . Unlike MET extract, the n-hexane and dichloromethane extracts showed toxic effects in cell suspensions. The MET extract significantly decreased cell death and ROS formation induced by IQ complex and copper and demonstrated protective effects against copper-induced mitochondrial membrane potential collapse and lipid peroxidation. The protection induced by MET extract can be attributed to antioxidant characteristics of phenylethanoids content

Full Text English
2 Downloads

Subscribe to VHSL Newsletters

© 2020 WHO – EMRO